Western blot abreview for Anti-beta Actin antibody [AC-15] (FITC)

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroglioma cells)
Gel Running Conditions
Reduced Denaturing (12%Acrylamide SDSPAGE)
Loading amount
100 µg
Treatment
0.5%triton
Specification
neuroglioma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/1000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Typhoon scan : laser:488nm emission filt
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 42kDa kDa

Additional data

Additional Notes
Two negative controls (BL21star E.coli cell lysate and purified protein TEV from E.coli) were used in additional Western blot.

Ms. Samira Acajjaoui

Verified customer

Submitted Aug 29 2016

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