Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig, Carp, Monkey Predicted to work with:
a wide range of other speciesDoes not react with:
Drosophila melanogaster, Dictyostelium discoideum
Synthetic peptide corresponding to beta Actin (N terminal) conjugated to keyhole limpet haemocyanin. Sequence:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Western blot - Anti-beta Actin antibody [AC-15] (HRP) (ab49900)
All lanes : Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/50000 dilution
Lane 1 : A549 Lysate Lane 2 : HeLa Lysate Lane 3 : 3T3 Lysate Lane 4 : NRK Lysate Lane 5 : MDCK Lysate Lane 6 : MDBK Lysate Lane 7 : BHK Lysate Lane 8 : COS7 Lysate Lane 9 : CFB Lysate
Developed using the ECL technique.
Predicted band size: 42 kDa
Western blot - Anti-beta Actin antibody [AC-15] (HRP) (ab49900)Image from PLoS One. 2010 Aug 25;5(8):e12400. Fig 8, doi: 10.1371/journal.pone.0012400.
Proteins were loaded onto a 10% SDS-polyacrylamide gel. After gel electrophoresis, blots were subsequently probed with primary antibodies anti- IL-6 at 1:1000 (ab6672), anti- IL-17 at 1:3000, anti-TGF-β at 1:1000 (ab66043). For detection, horseradish peroxidase-conjugated secondary anti-rabbit antibody was used followed by enhanced chemiluminescence development.
Normalization of results was ensured by running parallel western blots with β-actin antibody at 1:25,000 (ab49900). The optical density was quantified using an image densitometer. The data are presented as a percentage of target protein relative to β-actin. A value of p<0.05 is considered significant.
Western blot - Anti-beta Actin antibody [AC-15] (HRP) (ab49900)This image is courtesy of an anonymous Abreview
Anti-beta Actin antibody [AC-15] (HRP) (ab49900) at 1/25000 dilution + Whole cell lysate of Human acute monocytic leukemia THP-1. at 10 µg
Zhou Q et al. Fingerprint analysis of Huolingshengji Formula and its neuroprotective effects in SOD1G93Amouse model of amyotrophic lateral sclerosis. Sci Rep8:1668 (2018).
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