Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
10 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/25000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa

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Submitted Mar 19 2010

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