Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (ParC5 rat parotid cell line)
Loading amount
50 µg
Specification
ParC5 rat parotid cell line
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 25°C

Other product details

Dilution
1/25000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: tbst + 0.1% milk

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Pierce Supersignal West Pico
Exposure
3 second(s)
Bands
Specific: 42 kDa

Abcam user community

Verified customer

Submitted Sep 28 2010

Sign up