Western blot abreview for Anti-beta Actin [AC-15] antibody (HRP)

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk
Dilution
1/20000

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Mouse IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Bands
Specific: 43 kDa
Exposure
3 second(s)

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Submitted Jan 08 2014

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