Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk (TBS-tween)
Dilution
1/20000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 42 kDa

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Submitted Feb 09 2016

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