Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Loading amount
20 µg
Specification
293T
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/30000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: none observed kDa

Additional data

Additional Notes
Excellent antibody gave a single band of the predicted MW

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Submitted Jun 15 2007

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