Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
30 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/25000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Supersignal West Pico
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 42 kDa

Additional data

Additional Notes
Excellent antibody

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Verified customer

Submitted Jun 21 2007

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