Western blot abreview for Anti-beta Actin antibody [AC-15] (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/20000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 21°C · Diluent: 5% Milk 0.1% Tween-20 in PBS

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 22 (faint) kDa

Additional data

Additional Notes
Excellent loading control antibody

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Submitted Sep 20 2007

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