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Mouse monoclonal [BA3R] to beta Actin (HRP)
Mouse, Rat, Rabbit, Chicken, Human
Synthetic peptide corresponding to Human beta Actin (N terminal).
Database link: P60709
HeLa, 293T, U2OS, COS7, NIH 3T3 and NRK cell lysates.
Shipped at 4°C. Store at +4°C.
Concentration information loading...
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Predicted molecular weight: 42 kDa.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Belongs to the actin family.
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Information by UniProt
Western blot - Anti-beta Actin antibody [BA3R] (HRP) (ab173838)
All lanes :
Anti-beta Actin antibody [BA3R] (HRP) (ab173838) at 1/1000 dilution
Lane 1 :
HeLa cell lysate
Lane 2 :
293T cell lysate
Lane 3 :
U2OS cell lysate
Lane 4 :
COS7 cell lysate
Lane 5 :
NIH 3T3 cell lysate
Lane 6 :
NRK cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size:
4-20% Tris-HCl polyacrylamide gel.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"