Overview

  • Product name

    Anti-beta Actin antibody [BA3R] (HRP)
    See all beta Actin primary antibodies
  • Description

    Mouse monoclonal [BA3R] to beta Actin (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human beta Actin (N terminal).
    Database link: P60709

  • Positive control

    • HeLa, 293T, U2OS, COS7, NIH 3T3 and NRK cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab173838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42 kDa.Can be blocked with Recombinant Human beta Actin protein (His tag) (ab240844).

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • All lanes : Anti-beta Actin antibody [BA3R] (HRP) (ab173838) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U2OS cell lysate
    Lane 4 : COS7 cell lysate
    Lane 5 : NIH 3T3 cell lysate
    Lane 6 : NRK cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 42 kDa



    4-20% Tris-HCl polyacrylamide gel.

References

This product has been referenced in:

  • Pan Y  et al. Bromodomain PHD-finger transcription factor promotes glioma progression and indicates poor prognosis. Oncol Rep 41:246-256 (2019). Read more (PubMed: 30542695) »
  • Wang T  et al. Effects of leukemia inhibitory factor receptor on the adipogenic differentiation of human bone marrow mesenchymal stem cells. Mol Med Rep 19:4719-4726 (2019). Read more (PubMed: 31059010) »
See all 4 Publications for this product

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