Recombinant Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)
![Western blot - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-467892-272085-wb.JPG "Western blot - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR057F] to beta Actin - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-beta Actin antibody [BLR057F] - BSA and Azide free
See all beta Actin primary antibodies -
Description
Rabbit monoclonal [BLR057F] to beta Actin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human beta Actin aa 1-50. The exact sequence is proprietary. (NP_001092.1).
Database link: P60709 -
Positive control
- IHC-P: Human ovartian carcinoma tissue. ICC: HeLa cells. WB: U2-OS, A549, K562, HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysates.
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General notes
ab272085 is the carrier-free version of ab252556.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Carrier free
Yes -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR057F -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
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| WB |
1/1000. Predicted molecular weight: 41 kDa.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 41 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Omim: 102630 Human
- SwissProt: P60709 Human
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
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Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)All lanes : Anti-beta Actin antibody [BLR057F] (ab252556) at 1/1000 dilution
Lane 1 : U2-OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 8 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
Lysates prepared using NETN lysis buffer.
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![Immunocytochemistry - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-2-ab252556343365antibetaActinantibodyBLR057FimmunocytochemistryHeLahuman.jpg)
Immunocytochemistry - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)FFPE HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin using ab252556 at 1/200 dilution in ICC/IF, followed by HRP conjugated Goat Anti-Rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-1-ab252556343366antibetaActinantibodyBLR057Fimmunohistochemistryovariancarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for beta Actin using ab252556 at 1/400 dilution in immunohistochemical analysis, followed by HRP conjugated. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)](https://www.abcam.com/ps/products/272/ab272085/Images/ab272085-3-benefits-of-recombinant-antibodies.png)
Anti-beta Actin antibody [BLR057F] - BSA and Azide free (ab272085)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272085 has not yet been referenced specifically in any publications.