Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
Key features and details
- Rabbit polyclonal to beta Actin - Cytoskeleton Marker
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-beta Actin antibody - Cytoskeleton Marker
See all beta Actin primary antibodies -
Description
Rabbit polyclonal to beta Actin - Cytoskeleton Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human beta Actin aa 1-100. The exact sequence is proprietary.
Database link: P60709 -
Positive control
- WB: Recombinant Human alpha smooth muscle Actin protein (ab114148), U-2 OS and SK-N-SH cell lysate. IHC-P: Human leiomyoma tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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| IHC-P |
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| IP |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
|
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
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IP
Use a concentration of 5 µg/ml. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 450133 Chimpanzee
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 100033878 Horse
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 414396 Pig
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)All lanes : Anti-beta Actin antibody - Cytoskeleton Marker (ab16039) at 1 µg/ml
Lane 1 : SK-N-SH (human neuroblastoma) whole cell lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : U-2 OS (human osteosarcoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.
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Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 µg of Rabbit polyclonal to beta Actin and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.
Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 42kDa; beta Actin
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (81)
ab16039 has been referenced in 81 publications.
- Zhang J et al. Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells. Int J Med Sci 18:207-215 (2021). PubMed: 33390789
- Hung YK et al. In vitro effects of velvet antler water extracts from Formosan Sambar deer and red deer on barrier integrity in Caco-2 cell. Int J Med Sci 18:1778-1785 (2021). PubMed: 33746595
- Kuwano A et al. Microcirculatory disturbance in acute liver injury. Exp Ther Med 21:596 (2021). PubMed: 33884034
- Ando H et al. A novel polyethylene glycol (PEG)-drug conjugate of Venetoclax, a Bcl-2 inhibitor, for treatment of acute myeloid leukemia (AML). Cancer Rep (Hoboken) N/A:e1485 (2021). PubMed: 34173723
- Chen Y et al. The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p. Gen Physiol Biophys 39:229-237 (2020). PubMed: 32525816