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  1. Link

    beta-actin-antibody-cytoskeleton-marker-ab16039.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

  • Datasheet
  • SDS
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Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
  • Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

Key features and details

  • Rabbit polyclonal to beta Actin - Cytoskeleton Marker
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (ab115777)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-beta Actin antibody - Cytoskeleton Marker
    See all beta Actin primary antibodies
  • Description

    Rabbit polyclonal to beta Actin - Cytoskeleton Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human beta Actin aa 1-100. The exact sequence is proprietary.
    Database link: P60709

  • Positive control

    • WB: Recombinant Human alpha smooth muscle Actin protein (ab114148), U-2 OS and SK-N-SH cell lysate. IHC-P: Human leiomyoma tissue.
  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Actin protein (His tag) (ab240844)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP
Use a concentration of 5 µg/ml.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IP
Use a concentration of 5 µg/ml.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 450133 Chimpanzee
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 100033878 Horse
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 414396 Pig
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 443052 Sheep
    • Entrez Gene: 398459 Xenopus laevis
    • Entrez Gene: 407952 Xenopus tropicalis
    • Entrez Gene: 57934 Zebrafish
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: Q5R1X3 Chimpanzee
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60708 Horse
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: Q6QAQ1 Pig
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • SwissProt: O93400 Xenopus laevis
    • SwissProt: Q6NVA9 Xenopus tropicalis
    • SwissProt: Q7ZVI7 Zebrafish
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    • Unigene: 4138 Xenopus laevis
    • Unigene: 35143 Zebrafish
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
    Western blot - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
    All lanes : Anti-beta Actin antibody - Cytoskeleton Marker (ab16039) at 1 µg/ml

    Lane 1 : SK-N-SH (human neuroblastoma) whole cell lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : U-2 OS (human osteosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

    Formalin-fixed, paraffin-embedded human leiomyoma tissue stained for beta Actin with ab16039 (1/100 dilution) in immunohistochemical analysis.

  • Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)
    Immunoprecipitation - Anti-beta Actin antibody - Cytoskeleton Marker (ab16039)

    beta Actin was immunoprecipitated using 0.5mg SK-N-SH (human neuroblastoma cell line) whole cell extract, 5 µg of Rabbit polyclonal to beta Actin and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10 minutes, SK-N-SH whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16039.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 42kDa; beta Actin

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (81)

Publishing research using ab16039? Please let us know so that we can cite the reference in this datasheet.

ab16039 has been referenced in 81 publications.

  • Zhang J  et al. Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells. Int J Med Sci 18:207-215 (2021). PubMed: 33390789
  • Hung YK  et al. In vitro effects of velvet antler water extracts from Formosan Sambar deer and red deer on barrier integrity in Caco-2 cell. Int J Med Sci 18:1778-1785 (2021). PubMed: 33746595
  • Kuwano A  et al. Microcirculatory disturbance in acute liver injury. Exp Ther Med 21:596 (2021). PubMed: 33884034
  • Ando H  et al. A novel polyethylene glycol (PEG)-drug conjugate of Venetoclax, a Bcl-2 inhibitor, for treatment of acute myeloid leukemia (AML). Cancer Rep (Hoboken) N/A:e1485 (2021). PubMed: 34173723
  • Chen Y  et al. The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p. Gen Physiol Biophys 39:229-237 (2020). PubMed: 32525816
View all Publications for this product

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