Anti-beta Actin antibody - Loading Control (ab8229)
Key features and details
- Goat polyclonal to beta Actin - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Cow, Dog, Human, Chinese hamster
- Isotype: IgG
Overview
-
Product name
Anti-beta Actin antibody - Loading Control
See all beta Actin primary antibodies -
Description
Goat polyclonal to beta Actin - Loading Control -
Host species
Goat -
Specificity
This antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatRabbitChickenCowDogHumanChinese hamster
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta Actin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available asab13772) -
Positive control
- WB: HeLa, NIH3T3, PC12 and CHOK1 whole cell lysates.
-
General notes
This antibody has been designed for use as a loading control and is ideal for this purpose.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
This antibody has been designed for use as a loading control and is ideal for this purpose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
-
Related Products
- Anti-SDHB antibody [21A11AE7] (ab14714)
- Anti-SDHA antibody [2E3GC12FB2AE2] (ab14715)
- Phalloidin-iFluor 488 Reagent (ab176753)
- Phalloidin-iFluor 647 Reagent (ab176759)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
- Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
Rat
Rabbit
Chicken
Cow
Dog
Human
Chinese hamster
|
| All applications |
Goat
Chicken
Fish
Monkey
a wide range of other species
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (11) |
1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).
|
| Notes |
|---|
|
WB
1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). |
Target
-
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
-
Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
-
Western blot - Anti-beta Actin antibody - Loading Control (ab8229)All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutesSecondary antibody - rabbit anti-goat HRP (ab6741)
-
Western blot - Anti-beta Actin antibody - Loading Control (ab8229)Lanes 1-4 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 5% BSA.)
Lanes 5-8 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 3% Milk.)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lanes 3 & 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lanes 4 & 8 : CHO-K1 cell lysate Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin (lanes 1-4) or 3% milk (lanes 5-8) before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using an anti-Goat antibody conjugated to HRP, and visualised using ECL development solution. A non-specific band at 50-kDa is observed on western blots of some of our batches of ab8229. We have found that milk blocking is more effective than BSA blocking in eliminating this non-specific binding.
Datasheets and documents
-
SDS download
-
Datasheet download
References (169)
ab8229 has been referenced in 169 publications.
- Wright CB et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117:2579-2587 (2020). PubMed: 31964819
- Zhu M et al. Chronic Hypoxia-Induced Microvessel Proliferation and Basal Membrane Degradation in the Bone Marrow of Rats Regulated through the IL-6/JAK2/STAT3/MMP-9 Pathway. Biomed Res Int 2020:9204708 (2020). PubMed: 32047820
- Oppezzo A et al. Microphthalmia transcription factor expression contributes to bone marrow failure in Fanconi anemia. J Clin Invest 130:1377-1391 (2020). PubMed: 31877112
- Geng S et al. Bioactive phospho-therapy with black phosphorus for in vivo tumor suppression. Theranostics 10:4720-4736 (2020). PubMed: 32308745
- Liu LH et al. Paeonol exerts anti-tumor activity against colorectal cancer cells by inducing G0/G1 phase arrest and cell apoptosis via inhibiting the Wnt/ß-catenin signaling pathway. Int J Mol Med 46:675-684 (2020). PubMed: 32626954