Anti-beta Actin antibody - Loading Control (ab8229)
- Datasheet
- References (124)
- Protocols
Overview
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Product nameAnti-beta Actin antibody - Loading Control
See all beta Actin primary antibodies -
DescriptionGoat polyclonal to beta Actin - Loading Control
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Host speciesGoat
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SpecificityThis antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The immunogen used for this product shares 77% homology with Gamma actin/actin cytoplasmic 2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Goat, Chicken, Cow, Dog, Human, Fish, Monkey, Chinese hamster
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human beta Actin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available asab13772) -
Positive control
- WB: HeLa, NIH3T3, PC12 and CHOK1 whole cell lysates.
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General notes
This antibody has been designed for use as a loading control and is ideal for this purpose.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.025% Sodium azide
Constituent: PBS
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us. -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesThis antibody has been designed for use as a loading control and is ideal for this purpose.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
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- Phalloidin-iFluor 647 Reagent (ab176759)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
- Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
- Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
Applications
Our Abpromise guarantee covers the use of ab8229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). |
Target
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FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
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Sequence similaritiesBelongs to the actin family.
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Post-translational
modificationsISGylated. -
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
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Database links
- Entrez Gene: 396526 Chicken
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody - Loading Control (ab8229)All lanes : Anti-beta Actin antibody - Loading Control (ab8229) at 1/1000 dilution
Lane 1 : HeLa whole cell (Human)
Lane 2 : 3T3 cell (Mouse)
Lane 3 : Rabbit Liver
Lane 4 : MDCK cell (Dog)
Lane 5 : EBTr cell (Cow)
Lane 6 : SL-29 cell (Chicken)
Lane 7 : CHO cell (Chinese Hamster)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutesSecondary antibody - rabbit anti-goat HRP (ab6741)
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Western blot - Anti-beta Actin antibody - Loading Control (ab8229)Lanes 1-4 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 5% BSA.)
Lanes 5-8 : Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml (Blocked with 3% Milk.)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 2 & 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lanes 3 & 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lanes 4 & 8 : CHO-K1 cell lysate Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin (lanes 1-4) or 3% milk (lanes 5-8) before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using an anti-Goat antibody conjugated to HRP, and visualised using ECL development solution. A non-specific band at 50-kDa is observed on western blots of some of our batches of ab8229. We have found that milk blocking is more effective than BSA blocking in eliminating this non-specific binding.
Protocols
References
This product has been referenced in:
- Gong G et al. Tanshinone IIA alleviates oxidative damage after spinal cord injury in vitro and in vivo through up-regulating miR-124. Life Sci 216:147-155 (2019). Read more (PubMed: 30468834) »
- He M et al. Propofol alleviates hypoxia-induced nerve injury in PC-12 cells by up-regulation of microRNA-153. BMC Anesthesiol 18:197 (2018). Read more (PubMed: 30579328) »
