Application
Western blot
Sample
Human Cell lysate - whole cell (human C4-2 cells)
Loading amount
20 µg
Specification
human C4-2 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 680LT Donkey anti-Goat IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680LT
Dilution
1/20000
Detection
Detection method
LI-COR Odyssey
Exposure
30 second(s)
Bands
Specific: 40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Nov 17 2011