Application
Western blot
Sample
Rat Cell lysate - whole cell (Mixed Hippocampal Culture)
Loading amount
100 µg
Specification
Mixed Hippocampal Culture
Treatment
24hrs
Gel Running Conditions
Non-reduced Denaturing (10% Tris HCl)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Abcam antibody:Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Feb 01 2008