Anti-beta Actin antibody [M008] (ab253283)
![Western blot - Anti-beta Actin antibody [M008] (ab253283)](https://www.abcam.com/ps/products/253/ab253283/Images/ab253283-343948-antibetaactinantibodym008-westernblot-human.jpg "Western blot - Anti-beta Actin antibody [M008] (ab253283)")
Key features and details
- Mouse monoclonal [M008] to beta Actin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-beta Actin antibody [M008]
See all beta Actin primary antibodies -
Description
Mouse monoclonal [M008] to beta Actin -
Host species
Mouse -
Specificity
ab253283 preferentially detects human beta Actin with only weak reactivity toward actins in rat, mouse, or rabbit.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human beta Actin. (Proprietary antigen related to human beta actin in MDA-MB-231 breast cancer cell line).
Database link: P60709 -
Positive control
- WB: HUVEC-CS, Jurkat, HeLa and LNCaP cell lysates. ICC/IF: MeWo cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
M008 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab253283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
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| WB |
1/1000. Predicted molecular weight: 41 kDa.
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| Notes |
|---|
|
ICC/IF
1/50. |
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WB
1/1000. Predicted molecular weight: 41 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
see all -
Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Anti-beta Actin antibody [M008] (ab253283)All lanes : Anti-beta Actin antibody [M008] (ab253283) at 1/1000 dilution
Lane 1 : HUVEC-CS cell lysate
Lane 2 : Rabbit spleen fibroblast cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : LNCaP (human prostate cancer cell line) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 6 : F9 (mouse embryonic testicular cancer cell line) cell lysate
Predicted band size: 41 kDaPrimary for 1 hour at room temperature in PBS/5% non-fat milk/0.045 Tween 20.
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![Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] (ab253283)](https://www.abcam.com/ps/products/253/ab253283/Images/ab253283-343949-antibetaactinantibodym008-immunocytochemistryimmunofluorescence-MeWo-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody [M008] (ab253283)Paraformaldehyde-fixed MeWo (human epidermoid carcinoma cell line) cells stained for beta Actin (red) using ab253283 at 1/50 dilution in ICC/IF. The antibody was detected using goat anti-mouse DyLight® 594.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab253283 has not yet been referenced specifically in any publications.