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  1. Link

    beta-actin-antibody-mabcam-8224-bsa-and-azide-free-ab178787.pdf

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Isotype/Loading Controls Loading Controls Beta Actin
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Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
  • Flow Cytometry - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)

Key features and details

  • Mouse monoclonal [mAbcam 8224] to beta Actin - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free
    See all beta Actin primary antibodies
  • Description

    Mouse monoclonal [mAbcam 8224] to beta Actin - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Recognises a single band at 42kD representing beta Actin.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Schizosaccharomyces pombe, Chinese hamster, Other species
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab13772)

  • Positive control

    • Flow Cyt: HeLa cells; IHC-P: Human colon tissue.
  • General notes

    Ab178787 is a PBS Obly version of ab8224.

    This clone works well as a loading control for Xenopus, Drosophila, S. cerevisiae and S.pombe. We recommend using ab8224 instead of ab8226 for these species.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 8224
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Beta Actin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Associated products

  • Alternative Versions

    • HRP Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab197277)
    • Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Immunizing Peptide (Blocking)

    • Human beta Actin peptide (ab13772)
  • Recombinant Protein

    • Recombinant Chinese hamster beta Actin protein (His tag) (ab236171)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use 1µg for 106 cells.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.
Notes
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use 1µg for 106 cells.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5%BSA. Incubate antibody in TBST for one hour or more.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P60709 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396526 Chicken
    • Entrez Gene: 280979 Cow
    • Entrez Gene: 403580 Dog
    • Entrez Gene: 60 Human
    • Entrez Gene: 11461 Mouse
    • Entrez Gene: 414396 Pig
    • Entrez Gene: 100009272 Rabbit
    • Entrez Gene: 81822 Rat
    • Entrez Gene: 398459 Xenopus laevis
    • Omim: 102630 Human
    • SwissProt: P60706 Chicken
    • SwissProt: P48975 Chinese hamster
    • SwissProt: P60712 Cow
    • SwissProt: O18840 Dog
    • SwissProt: P60709 Human
    • SwissProt: P60710 Mouse
    • SwissProt: Q6QAQ1 Pig
    • SwissProt: P29751 Rabbit
    • SwissProt: P60711 Rat
    • SwissProt: O93400 Xenopus laevis
    • Unigene: 520640 Human
    • Unigene: 708120 Human
    • Unigene: 727576 Human
    • Unigene: 328431 Mouse
    • Unigene: 391967 Mouse
    • Unigene: 94978 Rat
    • Unigene: 4138 Xenopus laevis
    see all
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)

    IHC image of ab8224 staining beta Actin in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8224, 1μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab8224).

  • Flow Cytometry - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
    Flow Cytometry - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)

    Overlay histogram showing HeLa cells stained with ab8224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8224, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab8224).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [mAbcam 8224] - BSA and Azide free (ab178787)

    IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8224, 3µg/ml overnight at +4°C. A goat anti-mouse HRP-conjugated secondary antibody (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine and sodium azide (ab8224).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (24)

Publishing research using ab178787? Please let us know so that we can cite the reference in this datasheet.

ab178787 has been referenced in 24 publications.

  • Chowdhury D  et al. A proteomic view of isoproterenol induced cardiac hypertrophy: prohibitin identified as a potential biomarker in rats. J Transl Med 11:130 (2013). WB ; Rat . PubMed: 23706090
  • Szilagyi Z  et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol 32:2099-109 (2012). WB ; Schizosaccharomyces pombe . PubMed: 22451489
  • McIsaac RS  et al. Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae. Mol Biol Cell 22:4447-59 (2011). PubMed: 21965290
  • Ferreira ME  et al. The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes. PLoS One 6:e21113 (2011). WB ; Saccharomyces cerevisiae . PubMed: 21698254
  • Ellis SJ  et al. In vivo functional analysis reveals specific roles for the integrin-binding sites of talin. J Cell Sci 124:1844-56 (2011). WB . PubMed: 21558413
View all Publications for this product

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