Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Non-experimental Cerebellum)
Specification
Non-experimental Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
This Ab gives seemingly identical positivity when compared with that of ab6276.
beta Actin appears to be particularly enriched not only in the glomeruli of the Granule cell layer (indicated by red arrowheads ) but also in Microglia (indicated by green arrowheads). All positive microglia appear to be ramified thus not presumed to be activated.
Abcam response
Many thanks for this feedback on ab8224 (beta Actin antibody [mAbcam 8224] - Loading Control).

Mr. Carl Hobbs

Verified customer

Submitted Apr 21 2010

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