Western blot abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bEnd.3)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
bEnd.3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 42 kDa

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Submitted Feb 26 2016

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