Western blot abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (N87 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
400000 cells
Specification
N87 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBST
Concentration
0.5 µg/ml

Secondary antibody

Dilution
1/5000
Name

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 42 kDa

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Submitted Jun 22 2016

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