Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole brain tissue sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.05% Tween-20
Specification
Whole brain tissue sections
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Block
Dilution
1/1000

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: AlexaFluor Anti-Mouse IgG (H+L) 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 546

Additional data

Additional Notes
Tissue paraffin-embedded and sectioned onto slides at 15 microns.

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Submitted Mar 31 2017

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