Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblasts)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblasts
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X PBS/1% BSA/0.3% Triton™ X-100
Dilution
1/200

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555

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Submitted Nov 20 2017

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