Western blot abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

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Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (oocyte lysate)
Gel Running Conditions
Reduced Denaturing (10% SDS)
Loading amount
15 µg
Specification
oocyte lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: peroxidase-conjugated goat anti-mouse and anti-rab
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Exposure
34 second(s)
Bands
Specific: 42 kDa Non-specific: 55 kDa
Positive control
much stronger signal with HEK 293 cell lysate (15µg), double bands at 42 and 55, nice single band at 42 kDa in xenopus oocytes (also 15 µg)

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Submitted Oct 31 2018

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