Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Actin antibody [mAbcam 8224] - Loading Control

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey serum in PBST
Dilution
1/50

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti-MS
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Green - beta Actin blue - nucleus, DAPI.

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Submitted Apr 02 2019

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