Western blot abreview for Anti-beta Actin [mAbcam 8226] antibody - Loading Control (HRP)

Excellent
Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (MEF)
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Diluent: 5%milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ecl
Bands
Specific: around 45 kDa
Exposure
2 minute(s) and 0 second(s)

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Submitted Jan 02 2014

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