Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spleen)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-Tween 0.1%
Dilution
1/20000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Clarity™ Western ECL Substrate
Exposure
5 second(s)
Bands
Specific: 42 kDa

Additional data

Additional Notes
Lanes 1 and 2: Mouse spleen (whole tissue lysate), 2 different samples

Dr. Sergi Bayod

Verified customer

Submitted Mar 24 2017

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