Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP)

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS with 0.1% Blocking
Dilution
1/500

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Chemiluminescence
Exposure
25 second(s)
Bands
Specific: 43 kDa Non-specific: None kDa

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Submitted Dec 29 2017

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