Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP)

Below Average
Abreviews
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x TBS with 0.1% Blocking
Dilution
1/5000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
Chemiluminescence
Exposure
20 second(s)
Bands
Specific: 43 kDa

Additional data

Abcam response
We have not tested ab8226 with Macaca mulatta samples it however does give positive results with African green monkey samples; therefore, optimizations may be required to get expected results. As can be observed in image the product has performed well with human, mouse and rat samples.

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Verified customer

Submitted Dec 29 2017

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