Western blot abreview for Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP)

Good
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% MILK
Dilution
1/2500

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL
Negative control
NA
Exposure
30 second(s)
Bands
Specific: 40 kDa
Positive control
NA

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Submitted Mar 13 2019

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