Anti-beta Actin antibody - N-terminal (ab209869)
Key features and details
- Rabbit polyclonal to beta Actin - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-beta Actin antibody - N-terminal
See all beta Actin primary antibodies -
Description
Rabbit polyclonal to beta Actin - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Synthetic peptide within Zebrafish beta Actin aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Conjugated to a carrier protein.
Database link: Q7ZVI7 -
Positive control
- Adult Zebrafish eye, brain, ovary, heart and liver tissue extracts; Zebrafish tissue; whole Zebrafish extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 57934 Zebrafish
- SwissProt: Q7ZVI7 Zebrafish
- Unigene: 35143 Zebrafish
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Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling beta Actin with ab209869 at 1/300 dilution.
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All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/500 dilution
Lane 1 : Zebrafish heart tissue extract
Lane 2 : Whole Zebrafish tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa10% SDS-PAGE
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All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/1000 dilution
Lane 1 : Zebrafish eye tissue extract
Lane 2 : Zebrafish brain tissue extract
Lane 3 : Zebrafish ovary tissue extract
Lane 4 : Zebrafish liver tissue extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa10 % SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab209869 has been referenced in 2 publications.
- Hasanpour S et al. Maternal and zygotic activin signaling promotes adequate pattern and differentiation of mesoderm through regulation of pluripotency genes during zebrafish development. Int J Dev Biol 65:513-522 (2021). PubMed: 34549797
- Pakdaman Y et al. Chip Protein U-Box Domain Truncation Affects Purkinje Neuron Morphology and Leads to Behavioral Changes in Zebrafish. Front Mol Neurosci 14:723912 (2021). PubMed: 34630034