Overview

  • Product name

    Anti-beta Actin antibody - N-terminal
    See all beta Actin primary antibodies
  • Description

    Rabbit polyclonal to beta Actin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Synthetic peptide within Zebrafish beta Actin aa 2-65 (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: Q7ZVI7

  • Positive control

    • Adult Zebrafish eye, brain, ovary, heart and liver tissue extracts; Zebrafish tissue; whole Zebrafish extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab209869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

    • Alternative names

      • A26C1A antibody
      • A26C1B antibody
      • ACTB antibody
      • ACTB_HUMAN antibody
      • Actin beta antibody
      • Actin cytoplasmic 1 antibody
      • Actin, cytoplasmic 1, N-terminally processed antibody
      • Actx antibody
      • b actin antibody
      • b-actin antibody
      • Beta cytoskeletal actin antibody
      • Beta-actin antibody
      • BRWS1 antibody
      • E430023M04Rik antibody
      • Melanoma X actin antibody
      • MGC128179 antibody
      • PS1TP5 binding protein 1 antibody
      • PS1TP5BP1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling beta Actin with ab209869 at 1/300 dilution.

    • All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/500 dilution

      Lane 1 : Zebrafish heart tissue extract
      Lane 2 : Whole Zebrafish tissue extract

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 42 kDa



      10% SDS-PAGE

    • All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/1000 dilution

      Lane 1 : Zebrafish eye tissue extract
      Lane 2 : Zebrafish brain tissue extract
      Lane 3 : Zebrafish ovary tissue extract
      Lane 4 : Zebrafish liver tissue extract

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 42 kDa



      10 % SDS-PAGE

    References

    ab209869 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab209869.
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