Key features and details
- Rabbit polyclonal to beta Actin - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Zebrafish
- Isotype: IgG
Product nameAnti-beta Actin antibody - N-terminal
See all beta Actin primary antibodies
DescriptionRabbit polyclonal to beta Actin - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Zebrafish
Synthetic peptide within Zebrafish beta Actin aa 2-65 (N terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: Q7ZVI7
- Adult Zebrafish eye, brain, ovary, heart and liver tissue extracts; Zebrafish tissue; whole Zebrafish extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab209869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Sequence similaritiesBelongs to the actin family.
Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling beta Actin with ab209869 at 1/300 dilution.
All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/500 dilution
Lane 1 : Zebrafish heart tissue extract
Lane 2 : Whole Zebrafish tissue extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 42 kDa
All lanes : Anti-beta Actin antibody - N-terminal (ab209869) at 1/1000 dilution
Lane 1 : Zebrafish eye tissue extract
Lane 2 : Zebrafish brain tissue extract
Lane 3 : Zebrafish ovary tissue extract
Lane 4 : Zebrafish liver tissue extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
10 % SDS-PAGE
ab209869 has not yet been referenced specifically in any publications.