Recombinant

Recombinant Anti-beta Actin antibody [RM112] - N-terminal (Biotin) (ab222760)

Overview

  • Product name

    Anti-beta Actin antibody [RM112] - N-terminal (Biotin)
    See all beta Actin primary antibodies
  • Description

    Rabbit monoclonal [RM112] to beta Actin - N-terminal (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    No cross reactivity with other isoforms of Actin.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity


    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to beta Actin (N terminal).

  • Positive control

    • ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: A431 (human epidermoid carcinoma cell line) cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab222760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • Sequence similarities

    Belongs to the actin family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Alternative names

    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • b-actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • Melanoma X actin antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Images

  • Anti-beta Actin antibody [RM112] - N-terminal (Biotin) (ab222760) at 1/1000 dilution + A431 cell lysate
  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (red) using ab222760 at a 1/200 dilution in ICC/IF. Nuclei counterstain DAPI (blue).

References

ab222760 has not yet been referenced specifically in any publications.

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