Recombinant Anti-beta Actin antibody [SP124] - BSA and Azide free (ab242387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat placenta tissue sections labeling beta Actin with ab115777 at 1/100 dilution (0.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution (0.34 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115777)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling beta Actin with ab115777 at 1/200 dilution (0.34 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with citrate sodium buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115777)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1:50 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242387)

Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115777).

Intracellular Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling beta Actin with purified ab115777 at 1/200 dilution (0.57 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242387)

 

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1:50 (2.28 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242387)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling beta Actin with purified ab115777 at 1/200 dilution (0.57 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242387)

 

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling beta Actin with purified ab115777 at 1/200 dilution (0.57 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242387)

 

Intracellular flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue) 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115777).