Western blot abreview for Anti-beta Amyloid 1-40 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (brain capillary membranes)
Loading amount
1 µg
Specification
brain capillary membranes
Treatment
Roche Diagnostics Complete Protease Inhibitor
Gel Running Conditions
Reduced Denaturing (Invitrogen NuPage Bis-Tris 4-12%, 1 mm x 15 wells)
Blocking step
Pierce Protein-free T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce Protein-Free T20 (TBS) Blocking

Secondary antibody

Name
Non-Abcam antibody was used: ImmunoPure Goat Anti-rabbit IgG (H+L) Peroxidase C
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
Thermo Scientific SuperSignal West Pico
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 4 (published) kDa

Additional data

PubMed ID
20101004: View

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Submitted Mar 05 2012

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