Western blot abreview for Anti-beta Amyloid 1-40 antibody

Average
Abreviews
Application
Western blot
Sample
Guinea pig Tissue lysate - other (Brain)
Gel Running Conditions
Reduced Denaturing (7.5% gel)
Loading amount
25 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/15000
Name
Non-Abcam antibody was used: Bio-rad
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 4.1 kDa

Additional data

Additional Notes
Antibody used for WB obtained nonspecific and specific bands. Heavy dark gradient was presented in background.
Abcam response
Thank you for sharing this data with us. ab12265 has not previously been tested in Guinea Pig and we would welcome feedback from other customers using this product in this species. If interested, please contact us to participate in our Abtrial program: www.abcam.com/abtrial

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Verified customer

Submitted Mar 23 2016

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