Overview

  • Product name
    Anti-beta Amyloid 37-42 antibody
  • Description
    Rabbit polyclonal to beta Amyloid 37-42
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to beta Amyloid 37-42 (C terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab34376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/3000.
WB 1/400. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).

1/1000 for analysis of the recombinant protein, 1/400 for tissue extracts or cell lysates.

Images

  • Anti-beta Amyloid 37-42 antibody (ab34376) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 28 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    The band observed at 85 kDa could potentially be a cleaved form of beta Amyloid 37-42 due to the presence of a 17 amino acid signal peptide.

References

This product has been referenced in:
  • Lue LF  et al. Amyloid Beta and Tau as Alzheimer's Disease Blood Biomarkers: Promise From New Technologies. Neurol Ther 6:25-36 (2017). Read more (PubMed: 28733956) »
  • Zenaro E  et al. Neutrophils promote Alzheimer's disease-like pathology and cognitive decline via LFA-1 integrin. Nat Med N/A:N/A (2015). Sandwich ELISA ; Mouse . Read more (PubMed: 26214837) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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