Key features and details
- Rabbit polyclonal to beta B1 Crystallin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-beta B1 Crystallin antibody
DescriptionRabbit polyclonal to beta B1 Crystallin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Recombinant fragment corresponding to Mouse beta Crystallin A3 aa 6-186. N -terminal GST-tag and HIs-tag. Expressed in E.coli.
KASATTAVNPGPDGKGKGAPSTGPAPAPGPTPVPASVPRPAAKVGDLPPG SYRLIVFEQENFQGRRVEFSGECLNLGDRGFDRVRSLIVVSGPWVAFEQS AFRGEMFVLEKGEYPRWDTWTSSYRSDRLMSFRPIRMDSQEHKICLFEGA NFKGNTMEIQEDDVPSLWVYGFCDRVGSITV
Database link: Q9WVJ5
- WB: Recombinant mouse beta B1 Crystallin protein; Rat and mouse eye lysates. IHC-P: Mouse liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232799 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 28 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionCrystallins are the dominant structural components of the vertebrate eye lens.
Involvement in diseaseDefects in CRYBB1 are the cause of cataract congenital nuclear autosomal recessive type 3 (CATCN3) [MIM:611544]. A congenital cataract affecting the central nucleus of the eye. Nuclear cataracts are often not highly visually significant. The density of the opacities varies greatly from fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral. Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear opacity, which are referred to as cortical riders.
Defects in CRYBB1 are a cause of cataract-microcornea syndrome (CAMIS) [MIM:116150]. An ocular disorder characterized by the association of congenital cataract and microcornea without any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most often bilateral posterior polar with opacification in the lens periphery. The cataract progresses to form a total cataract after visual maturity has been achieved, requiring cataract extraction in the first to third decade of life. Microcornea-cataract syndrome can be associated with other rare ocular manifestations, including myopia, iris coloboma, sclerocornea and Peters anomaly. Transmission is in most cases autosomal dominant, but cases of autosomal recessive transmission have recently been described.
Sequence similaritiesBelongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains.
DomainHas a two-domain beta-structure, folded into four very similar Greek key motifs.
modificationsSpecific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization.
- Information by UniProt
- Beta crystallin B1 antibody
- Beta-B1 crystallin antibody
- Beta-crystallin B1 antibody
Anti-beta B1 Crystallin antibody (ab232799) at 2 µg/ml + Recombinant mouse beta B1 Crystallin protein
Predicted band size: 28 kDa
All lanes : Anti-beta B1 Crystallin antibody (ab232799) at 0.5 µg/ml
Lane 1 : Mouse eye lysate
Lane 2 : Rat eye lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/1000 dilution
Predicted band size: 28 kDa
Formalin-fixed, paraffin-embedded mouse liver tissue stained for beta B1 Crystallin using ab232799 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232799 has not yet been referenced specifically in any publications.