• Product name

    Anti-beta Bax antibody
  • Description

    Sheep polyclonal to beta Bax
  • Host species

  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 172-199 of Human beta Bax.

  • Positive control

    • in vitro translated Bax beta


Associated products


Our Abpromise guarantee covers the use of ab16143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes

    IP: Use at an assay dependent dilution.

    Is unsuitable for WB. Will not detect denatured protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • Tissue specificity

      Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Domain

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • Cellular localization

      Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Apoptosis regulator BAX antibody
      • Apoptosis regulator Bax cytoplasmic isoform beta antibody
      • BAX antibody
      • Bax B antibody
      • Bax beta antibody
      • BAX_HUMAN antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein isoform beta antibody
      • Bcl2-L-4 antibody
      see all


    • Immunoprecipitation of in vitro translated Bax alpha (1) and Bax beta (2) using Bax antibody clone 6A7, Bax alpha native (3) and denatured protein(4) using Bax beta antibody ab16143, and Bax beta native (5) and denatured (6) protein using Bax beta antibody ab16143. Ab16143 immunoprecipitated Bax beta strongly however did not detect Bax beta by western blotting.


    ab16143 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    I was informed that this antibody was not tested in house and therefore we unfortunately do not have an in house protocol for it. I would recommend the following general IP protocol which should work with ab16143 and can be found on our website: https://www.abcam.com/assets/pdf/protocols/Immunoprecipitation%20protocol%20(IP).pdf Please do not hesitate to contact me if I can be of help,

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    The antibody cannot detect the protein by western when the protein has been reduced because the reduction process opens up the protein which was in 3D and it becomes all unraveled. Some antibodies recognise an epitope which is a 3D part and therefore once the protein is reduced they cannot recognise the unraveled protein. The antibody ab16143 will recognise 3D native protein, which is why it works in IP (and will work in native western blotting). Other antibodies will recognise other parts of the protein which may not need to be in 3D form and therefore they will work in reduced western blotting (eg 6A7). Here is a guide which may help you: https://www.abcam.com/assets/pdf/protocols/WB-beginner.pdf I hope this clarification helps. Please do not hesitate to write to me if you need further help with your protocols or information on western blotting, I would be happy to help you,

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