Product nameAnti-beta Casein antibody [EPR12179]
See all beta Casein primary antibodies
DescriptionRabbit monoclonal [EPR12179] to beta Casein
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human beta Casein aa 100-200. The exact sequence is proprietary.
Database link: P05814
- Human milk lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab205301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).|
FunctionImportant role in determination of the surface properties of the casein micelles.
Tissue specificityMammary gland specific. Secreted in milk.
Sequence similaritiesBelongs to the beta-casein family.
modificationsForm 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25.
- Information by UniProt
- Beta-casein antibody
- CASB antibody
- CASB_HUMAN antibody
All lanes : Anti-beta Casein antibody [EPR12179] (ab205301) at 1/1000 dilution
Lane 1 : Human milk lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 5 seconds
Blocking/Dilution buffer: 5% NFDM/TBST.
beta Casein was immunoprecipitated from 1mg of Human milk lysate with ab205301 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab205301 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.
Lane 1: Human milk lysate10 µg (Input). Lane 2: ab205301 IP in Human milk lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205301 in Human milk lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds
ab205301 has not yet been referenced specifically in any publications.