Recombinant
RabMAb

Recombinant Anti-beta Casein antibody [EPR12179] - BSA and Azide free (ab232662)

Overview

  • Product name

    Anti-beta Casein antibody [EPR12179] - BSA and Azide free
    See all beta Casein primary antibodies
  • Description

    Rabbit monoclonal [EPR12179] to beta Casein - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human beta Casein aa 100-200. The exact sequence is proprietary.
    Database link: P05814

  • Positive control

    • WB: Human milk lysate.
  • General notes

    Ab232662 is the carrier-free version of ab205301. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232662 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.

Target

  • Function

    Important role in determination of the surface properties of the casein micelles.
  • Tissue specificity

    Mammary gland specific. Secreted in milk.
  • Sequence similarities

    Belongs to the beta-casein family.
  • Post-translational
    modifications

    Form 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta-casein antibody
    • CASB antibody
    • CASB_HUMAN antibody
    • Casein beta antibody
    • CSN 2 antibody
    • Csn2 antibody
    see all

Images

  • beta Casein was immunoprecipitated from 1mg of Human milk lysate with ab205301 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab205301 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/10000 dilution.

    Lane 1: Human milk lysate10 µg (Input). Lane 2: ab205301 IP in Human milk lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205301 in Human milk lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

  • All lanes : Anti-beta Casein antibody [EPR12179] (ab205301) at 1/1000 dilution

    Lane 1 : Human milk lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 25 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205301).

References

ab232662 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232662.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up