Western blot abreview for Anti-beta Catenin antibody [12F7]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Loading amount
100 µg
Specification
MEF
Gel Running Conditions
Non-reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/25000

Detection

Detection method
Visualizer
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 95 kDa

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Submitted Mar 06 2008

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