Western blot abreview for Anti-beta Catenin antibody [12F7]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney cancer cell)
Loading amount
20 µg
Specification
Kidney cancer cell
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: Dako Poly Rabbit Anti-Mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 95 kDa

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Submitted Nov 11 2010

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