Immunohistochemistry (Frozen sections) abreview for Anti-beta Catenin antibody [15B8]

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
Yes - PBST
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HISS/PBS (BLOCKING SOLUTION)
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: IgG Alexa Fluor 488 code number: 715-545-150 Jacks
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Ab-trail COD: 95191 I CHECKED IT WITH TWO TISSUE PREPARATION: Fix with PFA 4%, end oct freezing OR Fresh frozen in oct. Antibodies were examined in two protocols: Antigen retrieval protocol and Non Antigen retrieval. results: the staining was not specific for the tissue, all background.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Submitted Jan 20 2017

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