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    beta-catenin-antibody-ab16051.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Validated using a knockout cell line

Anti-beta Catenin antibody (ab16051)

  • Datasheet
  • SDS
Reviews (30)Q&A (1)References (144)

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Western blot - Anti-beta Catenin antibody (ab16051)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
  • Western blot - Anti-beta Catenin antibody (ab16051)
  • Western blot - Anti-beta Catenin antibody (ab16051)
  • Sandwich ELISA - Anti-beta Catenin antibody (ab16051)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

Key features and details

  • Rabbit polyclonal to beta Catenin
  • Suitable for: IHC-P, ICC/IF, Sandwich ELISA, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta Catenin antibody
    See all beta Catenin primary antibodies
  • Description

    Rabbit polyclonal to beta Catenin
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    sELISA
    Recombinant fragment
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human beta Catenin aa 750 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16377)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Catenin protein (Tagged) (ab63175)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • XAV939, Tankyrase inhibitor (ab120897)
    • Recombinant Human beta Catenin protein (Tagged) (ab63175)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
sELISA
Recombinant fragment
WB
Human
All applications
Mouse
Rat
Sheep
Goat
Chicken
Cat
Dog
Pig
Xenopus laevis
Zebrafish
Common marmoset
Squirrel
Dogfish
Catshark
Application Abreviews Notes
IHC-P (12)
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (6)
Use a concentration of 1 - 5 µg/ml.
Sandwich ELISA
Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [BDI080] to beta Catenin (ab19448) as Capture.
WB (7)
Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).
Notes
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Sandwich ELISA
Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [BDI080] to beta Catenin (ab19448) as Capture.
WB
Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

Target

  • Function

    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity

    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease

    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications

    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395964 Chicken
    • Entrez Gene: 477032 Dog
    • Entrez Gene: 1499 Human
    • Entrez Gene: 12387 Mouse
    • Entrez Gene: 397657 Pig
    • Entrez Gene: 84353 Rat
    • Entrez Gene: Sheep
    • Entrez Gene: 30265 Zebrafish
    • Omim: 116806 Human
    • SwissProt: B6V8E6 Dog
    • SwissProt: P35222 Human
    • SwissProt: Q02248 Mouse
    • SwissProt: Q9WU82 Rat
    • SwissProt: P26233 Xenopus laevis
    • Unigene: 476018 Human
    • Unigene: 291928 Mouse
    • Unigene: 112601 Rat
    see all
  • Alternative names

    • b-catenin antibody
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Images

  • Western blot - Anti-beta Catenin antibody (ab16051)
    Western blot - Anti-beta Catenin antibody (ab16051)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: CTNNB1 (β-catenin) knockout HAP1 whole cell lysate (20 µg)
    Lane 3: A431 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab16051 observed at 95 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab16051 was shown to specifically react with CTNNB1 (β-catenin) along with additional cross-reactive bands in wild-type HAP1 cells. No band was observed in knockout samples. Wild-type and CTNNB1 (β-catenin) knockout samples were subjected to SDS-PAGE. Ab16051 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 0.25 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

    ab16051 staining CTNNB1 (β-catenin) in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab16051 at 1μg/ml and ab7291 (staining Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

    IHC image of beta catenin staining in a formalin fixed, paraffin embedded normal human liver tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)

    IHC image of beta catenon staining in a formalin fixed, paraffin embedded human colon adenocarcinamo tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview by Carl Hobbs.

    ab16051 staining beta Catenin in rat brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

    ab16051 stained in MCF7cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16051 at 5 µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature

  • Western blot - Anti-beta Catenin antibody (ab16051)
    Western blot - Anti-beta Catenin antibody (ab16051)
    All lanes : Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate with Human beta Catenin peptide (ab16377) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 94 kDa
    Observed band size: 94 kDa
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.



    A second band of 75 kDa was also detected in Hela whole cell lysates and A431 lysates. This smaller band was of equal intensity to the 94 kDa band in the A431 lysates (data not shown).

  • Western blot - Anti-beta Catenin antibody (ab16051)
    Western blot - Anti-beta Catenin antibody (ab16051)
    Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml + Recombinant Human beta Catenin protein (Tagged) (ab63175) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 94 kDa


    Exposure time: 10 seconds
  • Sandwich ELISA - Anti-beta Catenin antibody (ab16051)
    Sandwich ELISA - Anti-beta Catenin antibody (ab16051)
    Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview by Carl Hobbs.

    ab16051 staining beta Catenin in developing gut tissue sections from mouse by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 2 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)

    ab16051 staining β-catenin in SW480 cells treated with XAV939 (ab120897), by ICC/IF. Increase of ß-catenin cytoplasmic expression and decrease in nuclear expression correlates with increased concentration of XAV939, as described in literature.

    The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120897 (XAV939) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview submitted by Miss Helen Gillingham

    ab16051 staining human fibrosarcoma cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to incubation with the primary antibody (at 10µg/ml) for 1 hour at 27°C.  A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols
  • Protocol Booklet - 1

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (144)

    Publishing research using ab16051? Please let us know so that we can cite the reference in this datasheet.

    ab16051 has been referenced in 144 publications.

    • Cui X  et al. Astragaloside IV suppresses development of hepatocellular carcinoma by regulating miR-150-5p/ß-catenin axis. Environ Toxicol Pharmacol 78:103397 (2020). PubMed: 32417721
    • Ji F  et al. The Circular RNA circRNA124534 Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Modulation of the miR-496/ß-Catenin Pathway. Front Cell Dev Biol 8:230 (2020). PubMed: 32318572
    • Chen ZF  et al. LncRNA SNHG20 enhances the progression of oral squamous cell carcinoma by regulating the miR-29a/DIXDC1/Wnt regulatory axis. Eur Rev Med Pharmacol Sci 24:5436-5445 (2020). PubMed: 32495922
    • Tang C  et al. Echinacoside inhibits breast cancer cells by suppressing the Wnt/ß-catenin signaling pathway. Biochem Biophys Res Commun 526:170-175 (2020). PubMed: 32201078
    • Yamazaki S  et al. The Actin-Family Protein Arp4 Is a Novel Suppressor for the Formation and Functions of Nuclear F-Actin. Cells 9:N/A (2020). PubMed: 32204557
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 31 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-beta Catenin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Pig Embryo (matured oocytes)
    Specification
    matured oocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Ferret Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EnVision FLEX Target Retrieval Solution
    Permeabilization
    No
    Specification
    Lung
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 15 2018

    IHC - Wholemount abreview for Anti-beta Catenin antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Tail bud 27 hpf)
    Specification
    Tail bud 27 hpf
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocyte (HaCaT))
    Permeabilization
    Yes - 0.2% Triton x100
    Specification
    Keratinocyte (HaCaT)
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ann Wheeler

    Verified customer

    Submitted May 17 2017

    Western blot abreview for Anti-beta Catenin antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human breast cancer cells)
    Gel Running Conditions
    Reduced Denaturing (Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl)
    Loading amount
    100 µg
    Specification
    human breast cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 23°C
    Read More

    Mrs. Katie Elliott

    Verified customer

    Submitted Aug 01 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Pluripotent stem cell)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    Pluripotent stem cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 04 2016

    Western blot abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human pluripotent stem cell)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    human pluripotent stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 14 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Marmoset (common) Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Myocardium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Myocardium
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 29 2016

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