Anti-beta Catenin antibody (ab6302)

Rabbit polyclonal beta Catenin antibody. Validated in WB, IHC, Dot, ICC/IF and tested in Mouse, Rat, Sheep, Horse, Chicken, Dog, Human, Pig, Xenopus laevis, Zebrafish, Botryllus schlosseri.

Overview

  • Product name
    Anti-beta Catenin antibody
    See all beta Catenin primary antibodies
  • Description
    Rabbit polyclonal to beta Catenin
  • Host species
    Rabbit
  • Specificity
    Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA. In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide. Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901). The antibody does not cross react with a-catenin or ?-catenin (plakoglobin).
  • Tested applications
    Suitable for: ICC/IF, Dot blot, IHC-Fr, WB, IHC-P, IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Horse, Chicken, Dog, Human, Pig, Xenopus laevis, Zebrafish, Botryllus schlosseri
  • Immunogen

    Synthetic peptide:

    PGDSNQLAWFDTDL

    conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse ß Catenin. (Peptide available as ab16377)

  • Positive control
    • MDBK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/2000. PubMed: 18624906
Dot blot 1/4000.
IHC-Fr 1/2000.
WB 1/4000. Predicted molecular weight: 94 kDa.
IHC-P Use at an assay dependent concentration.
IHC - Wholemount 1/200.

Target

  • Function
    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity
    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease
    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Images

  • All lanes : Anti-beta Catenin antibody (ab6302) at 1/4000 dilution

    Lane 1 : 5ug human lung tumour lysate.
    Lane 2 : 10ug human lung tumour lysate.
    Lane 3 : 20ug human lung tumour lysate.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H&L)HRP

    Performed under reducing conditions.

    Predicted band size: 94 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This image is courtesy of an Abreview submitted by Mike Campa on 4 April 2006.

    See Abreview

  • Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab6302 at 1/200. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 16 hours in PBS/0.05% Triton X 100 at 4 °C. An Alexa Fluor® 488 donkey anyti-rabbit was used as the secondary at 1/250.

    See Abreview

  • IHC-Wholemount image of anti-beta Catenin antibody (ab6302) staining on the Zebrafish jaw of a 5 day old larva. This larva was stained with anti-beta Catenin antibody (green) and a collagen 2 antibody (red) for 2 hours at 20°C. The image shows the cartilage elements of the jaw.

    See Abreview

  • This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.

     

  • ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.

    See Abreview

  • ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.

    See Abreview

References

This product has been referenced in:
  • Li XG  et al. Acute exposure to deoxynivalenol inhibits porcine enteroid activity via suppression of the Wnt/ß-catenin pathway. Toxicol Lett 305:19-31 (2019). Read more (PubMed: 30690062) »
  • Xu H  et al. Exosomes derived from PM2.5-treated lung cancer cells promote the growth of lung cancer via the Wnt3a/ß-catenin pathway. Oncol Rep 41:1180-1188 (2019). Read more (PubMed: 30431139) »
See all 119 Publications for this product

Customer reviews and Q&As

Question
Answer

Product Anti-beta Catenin antibody (ab6302) is provided in whole antiserum format, comprised of IgGs as well as other proteins. As this is not a purified format, an exact concentration of antigen-specific IgGs is not available.

The total IgG concentration in whole antiserum is between 1-10 mg/mL; the antigen-specific IgG concentration in whole antiserum is generally less than 0.5 mg/mL.

Read More

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