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  1. Link

    beta-catenin-antibody-ctnnb12030r-ab237984.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Recombinant

Recombinant Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
  • Western blot - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
  • Protein Array - Anti-beta Catenin antibody (ab237984)
  • Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CTNNB1/2030R] to beta Catenin
  • Suitable for: WB, IHC-P, Protein Array
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human beta Catenin protein (Tagged) (ab63175)

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Overview

  • Product name

    Anti-beta Catenin antibody [CTNNB1/2030R]
    See all beta Catenin primary antibodies
  • Description

    Rabbit monoclonal [CTNNB1/2030R] to beta Catenin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human beta Catenin.
    Database link: P35222

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human tonsil and cervical carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    CTNNB1/2030R
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-beta Catenin antibody [CTNNB1/2030R] - BSA and Azide free (ab237826)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human beta Catenin protein (Tagged) (ab63175)
  • Related Products

    • Recombinant Human beta Catenin protein (ab158230)
    • Recombinant Human beta Catenin protein (Tagged) (ab63175)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab237984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Protein Array
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 85 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Primary incubation for 30 minutes at room temperature.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.

Protein Array
Use at an assay dependent concentration.

Target

  • Function

    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity

    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease

    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications

    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1499 Human
    • Omim: 116806 Human
    • SwissProt: P35222 Human
    • Unigene: 476018 Human
    • Alternative names

      • b-catenin antibody
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)

      Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for beta Catenin using ab237984 at 2 μg/ml in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for beta Catenin using ab237984 at 2 μg/ml in immunohistochemical analysis.

    • Western blot - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
      Western blot - Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
      Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Predicted band size: 85 kDa

    • Protein Array - Anti-beta Catenin antibody (ab237984)
      Protein Array - Anti-beta Catenin antibody (ab237984)
      ab237984 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)
      Anti-beta Catenin antibody [CTNNB1/2030R] (ab237984)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab237984? Please let us know so that we can cite the reference in this datasheet.

    ab237984 has been referenced in 1 publication.

    • Wu CE  et al. Cinnamaldehyde enhances apoptotic effect of oxaliplatin and reverses epithelial-mesenchymal transition and stemnness in hypoxic colorectal cancer cells. Exp Cell Res 383:111500 (2019). PubMed: 31306656

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