Overview

  • Product name
    Anti-beta Catenin antibody [SP328] - BSA and Azide free
    See all beta Catenin primary antibodies
  • Description
    Rabbit monoclonal [SP328] to beta Catenin - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide within Human beta Catenin (C terminal). The exact sequence is proprietary.
    Database link: P35222

  • Positive control
    • IHC-P: Human breast ductal carcinoma, breast, bladder, bladder transitional carcinoma, stomach, colon, colon adenocarcinoma, kidney, renal cell carcinoma, liver, hepatocellular carcinoma, lung, lung squamous cell carcinoma, lung adenocarcinoma, ovary, ovary adenocarcinoma, prostate, prostate adenocarcinoma and stomach adenocarcinoma tissues. Flow Cyt: HEK-293 cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab242424 is a PBS-only buffer format of ab224803. Please refer to ab224803 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties

Applications

Our Abpromise guarantee covers the use of ab242424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Flow Cyt Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C

Target

  • Function
    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity
    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease
    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224803 for 10 mins at room temperature.

    This image was generated using ab224803, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the rat pancreas, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224803 for 10 mins at room temperature.

    This image was generated using ab224803, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse pancreas tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous staining on the mouse pancreas, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224803 for 10 mins at room temperature.

    This image was generated using ab224803, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling beta Catenin with ab224803 at 1/100 dilution (1.20 μg/ml). Heat mediated antigen retrieval with sodium Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly membranous with nuclear staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224803 for 10 mins at room temperature.

    This image was generated using ab224803, the same clone, but with a different buffer formulation.

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human ovary adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human ovary tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human lung squamous carcinoma tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human lung tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for beta Catenin withab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human colon tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human bladder transitional carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human bladder tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human breast tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Flow cytometric analysis of HEK-293 (human epithelial cell line from embryonic kidney) cell line labeling beta Catenin with ab224803 at 1/400 dilution (green) compared to a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

  • Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for beta Catenin with ab224803 at 1/400 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab224803).

References

ab242424 has not yet been referenced specifically in any publications.

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