Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37)
- Suitable for: WB, Dot blot
- Reacts with: Rat, Human
Product nameAnti-beta Catenin (phospho S37) antibody [EP742(2)Y]
See all beta Catenin primary antibodies
DescriptionRabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37)
Specificityab75777 detects beta Catenin phosphorylated on serine 37.
Tested applicationsSuitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human beta Catenin. The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding Serine 33 and 37 of human beta Catenin
- 293T cell lysates, untreated or treated with calyculin A.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).|
FunctionKey dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Tissue specificityExpressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
Involvement in diseaseDefects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Sequence similaritiesBelongs to the beta-catenin family.
Contains 12 ARM repeats.
modificationsPhosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
- Information by UniProt
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/5000 dilution
Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates
Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates treated with MG-132.
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates treated with MG-132 then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Blocking buffer 5% NFDM/TBST
Diluting buffer 5% NFDM/TBST
Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST.
Lane 1: Beta catenin (pS33+pS37) phospho peptide
Lane 2: Beta catenin (pS33) phospho peptide
Lane 3: Beta catenin (pS37) phospho peptide
Lane 4: Beta catenin non-phospho peptide
All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/500 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
ab75777 has been referenced in 6 publications.
- Jiang Z et al. Inactivation of the Wnt/ß-catenin signaling pathway underlies inhibitory role of microRNA-129-5p in epithelial-mesenchymal transition and angiogenesis of prostate cancer by targeting ZIC2. Cancer Cell Int 19:271 (2019). PubMed: 31649488
- He J et al. Sox2 inhibits Wnt-ß-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells. Mol Med Rep 15:1693-1701 (2017). WB ; Human . PubMed: 28259951
- Liang C et al. MicroRNA-433 inhibits cervical cancer progression by directly targeting metadherin to regulate the AKT and ß-catenin signalling pathways. Oncol Rep 38:3639-3649 (2017). PubMed: 29130111
- Zhang J et al. SOX4 inhibits GBM cell growth and induces G0/G1 cell cycle arrest through Akt-p53 axis. BMC Neurol 14:207 (2014). WB ; Human . PubMed: 25366337
- Li WF et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). ICC/IF ; Human . PubMed: 24728101
- Qiu DZ et al. Bufalin, a component in Chansu, inhibits proliferation and invasion of hepatocellular carcinoma cells. BMC Complement Altern Med 13:185 (2013). WB ; Human . PubMed: 23870199