Recombinant Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
![Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)](https://www.abcam.com/ps/products/247/ab247399/Images/ab247399-405515-anti-beta-catenin-phospho-s37-antibody-ep742-2-y-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free
See all beta Catenin primary antibodies -
Description
Rabbit monoclonal [EP742(2)Y] to beta Catenin (phospho S37) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects beta Catenin phosphorylated on serine 37.
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Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human beta Catenin. The exact sequence is proprietary. A phospho specific peptide corresponding to residues surrounding Serine 33 and 37 of human beta Catenin
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General notes
ab247399 is the carrier-free version of ab75777 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP742(2)Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
Target
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Function
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
Tissue specificity
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. -
Involvement in disease
Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
Sequence similarities
Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
Post-translational
modificationsPhosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 1499 Human
- Entrez Gene: 84353 Rat
- Omim: 116806 Human
- SwissProt: P35222 Human
- SwissProt: Q9WU82 Rat
- Unigene: 476018 Human
- Unigene: 112601 Rat
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Alternative names
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
see all
Images
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Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/5000 dilution
Lane 1 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate treated with MG-132.
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate treated with MG-132 then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab75777, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)](https://www.abcam.com/ps/products/247/ab247399/Images/ab247399-2-anti-beta-catenin-phospho-s37-antibody-ep742-2-y-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)This data was developed using ab75777, the same antibody clone in a different buffer formulation.Dot blot analysis of Beta catenin phospho peptide with ab75777 at 1/1000 exposed for 3 minutes. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Blocking buffer 5% NFDM/TBST. Diluting buffer 5% NFDM/TBST. Lane 1: Beta catenin (pS33+pS37) phospho peptide
Lane 2: Beta catenin (pS33) phospho peptide
Lane 3: Beta catenin (pS37) phospho peptide
Lane 4: Beta catenin non-phospho peptide -
![Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)](https://www.abcam.com/ps/products/247/ab247399/Images/ab247399-397925-anti-beta-catenin-phospho-s37-antibody-ep742-2-y-bsa-and-azide-free-western-blot.gif)
Western blot - Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)All lanes : Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] (ab75777) at 1/500 dilution
Lane 1 : 293T cell lysates, untreated
Lane 2 : 293T cell lysates, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaThis data was developed using ab75777, the same antibody clone in a different buffer formulation.
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![Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)](https://www.abcam.com/ps/products/247/ab247399/Images/ab247399-4-benefits-of-recombinant-antibodies.png)
Anti-beta Catenin (phospho S37) antibody [EP742(2)Y] - BSA and Azide free (ab247399)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247399 has not yet been referenced specifically in any publications.
