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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-beta Catenin (phospho Y142) antibody (ab27798)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (37)

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Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)

Key features and details

  • Rabbit polyclonal to beta Catenin (phospho Y142)
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta Catenin (phospho Y142) antibody
    See all beta Catenin primary antibodies
  • Description

    Rabbit polyclonal to beta Catenin (phospho Y142)
  • Host species

    Rabbit
  • Specificity

    The antibody detects beta Catenin on SDS PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate treated cell lines, such as A431 and human endothelial cells. Immunogen sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma Catenin. These human sequences are highly conserved in rat and mouse beta Catenins. Batches could vary in the level phospho Y133 of gamma catenin recognition.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    See all applications and species data
  • Immunogen

    Phospho beta Catenin (Tyr 142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta Catenin.

  • General notes

    Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Tyrosine phosphorylation of beta Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro and overexpression of these kinases in epithelial cells disrupts interactions between alpha and beta Catenins. The phosphorylation of tyrosine 142 may act as a switch from the adhesive to the transcriptional role of beta Catenin. Thus, site specific tyrosine phosphorylation of beta Catenin may regulate specific protein protein interactions, leading to changes in cell adhesion.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Catenin protein (Tagged) (ab63175)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Application Abreviews Notes
WB (3)
1/500. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa). IP with beta catenin antibody is recommended, followed by probing with ab27798. This improve the distinction between beta Catenin Tyr 142 and gamma Catenin Tyr 133 (Swiss-Prot ID P14923).
ICC/IF (1)
1/50.
Notes
WB
1/500. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa). IP with beta catenin antibody is recommended, followed by probing with ab27798. This improve the distinction between beta Catenin Tyr 142 and gamma Catenin Tyr 133 (Swiss-Prot ID P14923).
ICC/IF
1/50.

Target

  • Function

    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • Tissue specificity

    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • Involvement in disease

    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities

    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • Post-translational
    modifications

    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 12387 Mouse
    • SwissProt: Q02248 Mouse
    • Unigene: 291928 Mouse
    • Alternative names

      • b-catenin antibody
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    Images

    • Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)
      Western blot - Anti-beta Catenin (phospho Y142) antibody (ab27798)
      Lanes 1-2 : anti beta catenin
      Lanes 3-4 : Anti-beta Catenin (phospho Y142) antibody (ab27798) at 1/500 dilution

      Lanes 1 & 3 : Hct116 src transformed cells serum starved overnight.
      Lanes 2 & 4 : Hct116 src transformed cells treated with pervanadate (1 mM) for 30 min.

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 86 kDa



      Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
    • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)
      Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)

      ICC/IF labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal β-Catenin (left panel) and ab27798 antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

    • Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)
      Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin (phospho Y142) antibody (ab27798)Image courtesy of an Abreview submitted by Judit Herreros.

      ab27798 staining beta Catenin (phospho Y142) in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence. The cells were pre-treated with pervanadate for 15 minutes before being fixed in paraformaldehyde and then blocked using 10% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/50 for 8 hours at 4°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) ab150076) used at a 1/500 dilution. Hoestch was used to stain the cell nuclei (blue).

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (37)

    Publishing research using ab27798? Please let us know so that we can cite the reference in this datasheet.

    ab27798 has been referenced in 37 publications.

    • Zhang Q  et al. Long noncoding RNA MAGI1-IT1 regulates cardiac hypertrophy by modulating miR-302e/DKK1/Wnt/beta-catenin signaling pathway. J Cell Physiol 235:245-253 (2020). PubMed: 31222747
    • Shen E  et al. MicroRNA-93-5p promotes epithelial-mesenchymal transition in gastric cancer by repressing tumor suppressor AHNAK expression. Cancer Cell Int 20:76 (2020). PubMed: 32190000
    • Huo LW  et al. circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling. Mol Med 26:29 (2020). PubMed: 32268875
    • Du W  et al. MiR-495 targeting dvl-2 represses the inflammatory response of ankylosing spondylitis. Am J Transl Res 11:2742-2753 (2019). PubMed: 31217850
    • Liu YB  et al. Silencing long non-coding RNA MEG3 accelerates tibia fraction healing by regulating the Wnt/ß-catenin signalling pathway. J Cell Mol Med 23:3855-3866 (2019). PubMed: 30955246
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin (phospho Y142) antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (BRAIN)
    Permeabilization
    Yes - TRITON
    Specification
    BRAIN
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 01 2019

    Question

    For product Anti-beta Catenin (phospho Y142) antibody (ab27798), can you please provide the Pervanadate Treatment Protocol, as used in the WB image for this Ab?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 03 2012

    Answer

    Please see below for our protocol for the Pervanadate Treatment of Cells.

    Reagents:
    - Pervanadate (100mM stock)
    - 100uL 30% Hydrogen Peroxide (H2O2)
    - 200uL 500mM Sodium Vanadate
    - 700uL dH2O

    Serum Starvation and Cell Stimulations:
    1. Grow cells of interest to 80-90% confluency (adherent cells: A431, J774A.1, HUVEC, SYF, RSF, COS7, differentiated PC12) or medium to high cell density (suspension cells: Jurkat, K562, Sp2O, undifferentiated PC12) in culture dishes.

    2. Cell stimulations for studies of phosphorylation can examine serum starved cells to reduce basal phosphorylation levels prior to the addition of stimulants.

    3. For adherent cells, remove cell culture growth media and rinse cells once in serum-free containing media (usually DMEM or F12K). Next, add serum-free media to each dish and place cells in incubator for 2 hrs (most cell lines) or overnight (A431 only). After serum starvation, add stimulant (treated) or vehicle (control) at the concentration and time required: 1 mM Pervanadate in dH2O for 30 min. (Some cell lines may come off dish with this concentration).

    Read More

    Abcam Scientific Support

    Answered on Feb 03 2012

    Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (immunoprecipitated b-catenin from mES cells)
    Loading amount
    1e+006 cells
    Specification
    immunoprecipitated b-catenin from mES cells
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Dr. Penny Hayward

    Verified customer

    Submitted Jan 17 2012

    Immunocytochemistry abreview for Anti-beta Catenin (phospho Y142) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Rat Cultured Cells (Hippocampal neurons)
    Specification
    Hippocampal neurons
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Dr. Judit Herreros

    Verified customer

    Submitted Apr 01 2009

    Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela S3 cells)
    Loading amount
    30 µg
    Specification
    Hela S3 cells
    Treatment
    +/- [30 mM] Pervanadate for 30 min
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 01 2007

    Western blot abreview for Anti-beta Catenin (phospho Y142) antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (pancreas)
    Loading amount
    30 µg
    Specification
    pancreas
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Alessandra Strom

    Verified customer

    Submitted May 10 2006

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