Immunocytochemistry abreview for Anti-beta Catenin (phospho Y142) antibody

Good
Abreviews
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Hippocampal neurons)
Specification
Hippocampal neurons
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/50
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500

Additional data

PubMed ID
18664491: View
Additional Notes
Partial colocalization with nuclear staining using Hoestch. Require pervanadate pre-treatement for 15 min before fixing.

Dr. Judit Herreros

Verified customer

Submitted Apr 01 2009

Sign up